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Chapter 1 - The Polymerase Chain Reaction: PCR, qPCR, and RT-PCR
Mehdi Jalali, Justyna Zaborowska and Morteza Jalali
Pages 1-18 - Book chapterAbstract only
Chapter 2 - Methods of Cloning
Alessandro Bertero, Stephanie Brown and Ludovic Vallier
Pages 19-39 - Book chapterAbstract only
Chapter 3 - Whole-Mount In Situ Hybridization and a Genotyping Method on Single Xenopus Embryos
Martyna Lukoseviciute, Robert Lea, ... Enrique Amaya
Pages 41-56 - Book chapterAbstract only
Chapter 4 - Microarrays: An Introduction and Guide to Their Use
Frederick D. Park, Roman Sasik and Tannishtha Reya
Pages 57-76 - Book chapterAbstract only
Chapter 5 - Analysis of Human Genetic Variations Using DNA Sequencing
Gregory A. Hawkins
Pages 77-98 - Book chapterAbstract only
Chapter 6 - Western Blot
Tomasz Gwozdz and Karel Dorey
Pages 99-117 - Book chapterAbstract only
Chapter 7 - The Enzyme-Linked Immunosorbent Assay: The Application of ELISA in Clinical Research
Jefte M. Drijvers, Imad M. Awan, ... Shiv Pillai
Pages 119-133 - Book chapterAbstract only
Chapter 8 - Immunofluorescence
Sonali Joshi and Dihua Yu
Pages 135-150 - Book chapterAbstract only
Chapter 9 - Cell Culture: Growing Cells as Model Systems In Vitro
Charis-P. Segeritz and Ludovic Vallier
Pages 151-172 - Book chapterAbstract only
Chapter 10 - Flow Cytometry
Eleni Chantzoura and Keisuke Kaji
Pages 173-189 - Book chapterAbstract only
Chapter 11 - Transfection
Semira Sheikh, Amanda S. Coutts and Nicholas B. La Thangue
Pages 191-209 - Book chapterAbstract only
Chapter 12 - In Vivo Animal Modeling: Drosophila
Michael F. Wangler and Hugo J. Bellen
Pages 211-234 - Book chapterAbstract only
Chapter 13 - Zebrafish as a Research Organism: Danio rerio in Biomedical Research
John Collin and Paul Martin
Pages 235-261 - Book chapterAbstract only
Chapter 14 - Xenopus as a Model Organism for Biomedical Research
Shoko Ishibashi, Francesca Y.L. Saldanha and Enrique Amaya
Pages 263-290 - Book chapterAbstract only
Chapter 15 - Basic Mouse Methods for Clinician Researchers: Harnessing the Mouse for Biomedical Research
Laurens J. Lambert, Mandar D. Muzumdar, ... Tyler Jacks
Pages 291-312 - Book chapterNo access
Appendix A - Legal Framework on the Scientific Use of Animals in Research
David Pettitt, Adam Pettitt, ... David Brindley
Pages 313-326 - Book chapterNo access
Appendix B - Regulatory Frameworks for Stem Cell Research
Eleanor Jane Budge and Morteza Jalali
Pages 327-338 - Book chapterNo access
Appendix C - Using Multiple Experimental Methods to Address Basic Science Research Questions
Justyna Zaborowska and Morteza Jalali
Pages 339-343 - Book chapterNo access
Index
Pages 345-355
About the book
Description
Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data.
This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science.
Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data.
This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science.
Key Features
- Serves as a helpful guide for clinical researchers who lack a conventional science background
- Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms
- Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data
- Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
- Serves as a helpful guide for clinical researchers who lack a conventional science background
- Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms
- Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data
- Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Details
ISBN
978-0-12-803077-6
Language
English
Published
2016
Copyright
Copyright © 2016 Elsevier Inc. All rights reserved.
Imprint
Academic Press